Figure 3.

Potato JAMYC2 and JAMYC10 overexpressers show an enhanced induction of the LAP and pin2 genes upon MeJA treatment. (A) LAP and pin2 gene expression in wild-type controls (control) and the 35S:JAMYC2 and 35S:JAMYC10 transgenic potato plants after 6 h of treatment with increasing concentrations of MeJA. Each lane was loaded with 20 μg of total RNA. Blots were hybridized with the LAP, pin2, and JAMYC2 or JAMYC10 probes as indicated. Equal loading was verified by EtBr staining of the gel. (B) Histograms represent the levels of expression of LAP and pin2 as quantified by densitometric scanning of the blot.