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. 2004 Jul 1;18(13):1592–1605. doi: 10.1101/gad.1200204

Figure 1.

Figure 1.

Suz12 protein expression is elevated in human colon tumors. (A) Western blot analysis to demonstrate the specificity of the Suz12 antibody. SW480 cells were transfected with an HA-Suz12 or a control (HA-empty) construct and subjected to immunoblot analysis. Two separate blots were prepared and probed with either an HA antibody (left panel) or a Suz12 antibody (right panel). The HA antibody recognizes only the HA-Suz12 protein and the Suz12 antibody detects both the HA-Suz12 and endogenous Suz12 proteins. (B) Western blot analysis of whole-tissue extracts prepared from normal (N) and tumor (T) tissues of five different colon cancer patients. The blot was first probed with an anti-Suz12 antibody and then reprobed with an antiactin antibody as a loading control. The arrowhead points to the band with the correct size of Suz12 (93 kDa). A second lower band, detected with the Suz12 antibody in patients 1–3, probably represents a protein degradation product.