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. 2004 Jul 1;18(13):1592–1605. doi: 10.1101/gad.1200204

Figure 2.

Figure 2.

Suz12 expression in colon cancer cells is disrupted by RNA interference. (A) RT–PCR analysis on total RNA extracted from SW480 cells at various time points after transfection with Suz12 or GFP siRNA duplexes. Primers specific to the GAPDH mRNA were used in RT–PCR to ensure that the RNA was correctly quantitated. (B) Western blot analysis on whole-cell extracts prepared from SW480 cells at various time points after transfection with Suz12 or Lamin A/C siRNA duplexes. Antibodies against Suz12 and Lamin A/C were used to show specific depletion of the respective proteins. The blot was also probed with the actin antibody to demonstrate equal loading of protein samples.