Figure 4.

The MYT1 promoter is directly regulated by Suz12. Chromatin immunoprecipitation experiments were performed in SW480 cells using antibodies to RNA Polymerase II (lane 2), Suz12 (lane 3), and a control IgG (lane 4). A control immunoprecipitation was also performed with a Suz12 antibody that was preincubated with the Suz12 immunogen at a 10-fold excess by weight (lane 1). Three dilutions of the total input are also shown (lanes 5–7). The precipitated chromatin was analyzed with PCR using primers specific to the promoters of the indicated genes.