Figure 8.

Methylation of H3-K27 at the Suz12 target promoters is due to Suz12-mediated recruitment of the methyltransferase Ezh2. ChIP experiments were performed using antibodies to Suz12 (lanes 1,6), Ezh2 (lanes 2,7), trimethyl H3-K27 (lanes 3,8), and a control IgG (lanes 4,9) in SW480 cells that were incubated with siSuz12 smart-pool or no siRNA (mock). For the siRNA transfections, cells were incubated with the indicated siRNA for 72 h, then replated and transfected for another 72 h. The immunoprecipitated chromatin was analyzed by PCR using primers specific to the promoters of the genes indicated. An equal amount of DNA from both treatments was monitored using PCR as shown by the similar intensity of the two input signals (cf. lanes 6 and 12).