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. 2004 Jul 1;18(13):1606–1617. doi: 10.1101/gad.1193404

Figure 5.

Figure 5.

The MTE functions with the Inr in a spacing-dependent manner. (A) The Inr is required for transcription from MTE-containing promoters. In the mutant Inr (mInr) promoters, the Inr sequences (shown in Fig. 1) were mutated to GTGACA. The constructs were subjected to in vitro transcription analysis with a Drosophila nuclear extract. (B) The spacing between the Inr and the MTE is important for core promoter activity. A series of mutant promoters was constructed in which the spacing between the Inr and the MTE was either increased or decreased by one or three nucleotides, as depicted. To ensure that the effects are due to interactions between the Inr and MTE, all of the promoters contain the m30–33 mutation (CATA at +30–33) that inactivates the DPE motif. The constructs were subjected to in vitro transcription analysis with a Drosophila nuclear extract. The transcriptional activity of each mutant promoter is reported relative to that of the m30–33 promoter with wild-type spacing (0) between the Inr and the MTE.