FIG. 2.

Activity and expression of G6PDH are reduced in human ESC-Ts exposed to GlcN. Human ESC-Ts were collected after in vitro decidualization for 9 days in the presence of 0 or 5 mM GlcN. A) The levels of G6PDH activity were measured by microanalytic assay and normalized to cell counts. B) The levels of G6PDH expression were measured by quantitative real-time PCR. Data is expressed as mean ± SEM of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 compared with the decidualized sample. C) Western blot exhibits G6PDH protein expression and the loading control, ACTIN. Lower panel is the quantification of duplicate Western blots.