FIG. 4.

Decidualization is inhibited both in vitro and in vivo by GlcN in mice. A) Primary cultures of ESCs isolated from mice were decidualized with E₂ and P₄ in the absence or presence of GlcN for 3 days in vitro. Decidualization was assessed by monitoring the expression of Prp mRNA. Values are the mean of three independent experiments ± SEM. B) Representative uteri from mice in each treatment group—control and GlcN—are shown. The horn that received a physical stimulus to induce deciduoma formation is on the right, and the unstimulated internal control horn is on the left. C) The mass of deciduoma and control uterine horns was quantified for control and GlcN-treated mice. Values are the mean of three mice in each group ± SEM. *P < 0.05; **P < 0.01. D) Representative cross-section of the uterine horns stained with hematoxylin and eosin. Bar = 2 mm.