FIG. 1.

Effect of DMBA on ovarian weight and follicle numbers in lean and obese mice. At 18 wk of age, lean and obese mice were intraperitoneally (i.p) dosed with sesame oil or DMBA (95%; 1mg/kg) for 14 days. Mice were killed 3 days after the end of dosing; body weight was measured (A), and ovaries were collected, trimmed of excess fat, and weighed (B). One ovary was fixed in 4% paraformaldehyde, and complete serial sections were prepared and subjected to histological examination for the number of healthy follicles in both lean and obese treated groups (C–F); (C) healthy primordial follicle number; (D) healthy primary follicle number; (E) healthy secondary follicle number; and (F) healthy preovulatory follicle number. Bars represent mean follicle number per ovary ± SEM. Different letters indicate significant difference between respective pairs (P < 0.05; n = 3).