FIG. 5.

Effect of DMBA on ovarian PI3K proteins in lean and obese mice. Eighteen-wk-old lean and obese female mice were treated with sesame oil or DMBA (95%; 1mg/kg; i.p) for 14 days. Three days after dosing, ovaries were collected, trimmed of excess fat, and total ovarian protein was isolated (n = 3 per treatment group) from lean control (LC), lean DMBA (LD), obese control (OC) and obese DMBA (OD). Western blot analysis was performed to measure (A) pAKT^Ser473, (B) pAKT^Thr308, (C) FOXO3, and (D) pFOXO3^Ser253 levels. Densitometry of the appropriate bands was performed using Carestream molecular imaging software version 5.0. Bars represent means ± SEM. Different letters indicate significant difference from respective pairs (P < 0.05; n = 3).