Figure 12. Representative 12% SDS-PAGE gel image showing Proteinase-K digestion products of WtAS aggregated alone or in the presence of compounds 1, 3, 6 and 15.

WtAS (210 µM) were aggregated alone or co-incubated with equimolar concentration of compound 1, 3, 6 and 15 for 30 days. 20 µl WtAS samples were subjected to digestion by 50 µg/ml of Proteinase-K for 1 h at 37°C and boiled at 100°C for 5 min after adding SDS sample buffer. Lane 1-undigested, Lane 2–6 digested samples of only WtAS with 1, 3, 6, and 15.