Figure 3. (a) Time course of WtAS aggregation in the presence or absence of compounds 1–19 as monitored by ThT assay. WtAS (210 µM) was co-incubated with equimolar concentrations of curcumin (compound 1) or compounds 2–19 for 30 days. The ability of these compounds to prevent aggregation is shown as percentage increase in ThT fluorescence intensity. Aggregated samples were incubated with 50 µM of ThT for 30 min at 25°C. Samples were excited at 440 nm and emissions recorded at 480 nm. Results are the mean of three different experiments (n = 3) done in duplicate and the error bars show the standard deviations. # p < 0.005 compound 1 vs compound 3, compound 1 vs compound 15, $ p < 0.0001 compound 1 vs compound 6 (b) Percentage inhibition of aggregation of WtAS by compound 1–19 as monitored by ThT assay. WtAS (210 µM) was co-incubated with equimolar concentration of curcumin (compound 1) or compounds 2–19 for 30 days. The ability of these compounds to prevent aggregation is shown as percent inhibition of WtAS aggregation on day 30 as compared to WtAS alone. Aggregated samples were incubated with 50 µM of ThT for 30 min at 25 °C. Samples were excited at 440 nm and emissions recorded at 480 nm. Percent inhibition was calculated using appropriate equation. Results are the mean of three different experiments (n = 3) done in duplicate and the error bars show the standard deviations. # p < .005 compound 1 vs compound 3 and 15 and $ p < 0.0001 compound 1 vs compound 6.