Figure 4.

The spores of the mcp7Δ strain are abnormal. (A) Number of ascospores per ascus in the mcp7Δ, meu13Δ and rad17Δ single and double mutants. The diploid strains were induced to enter meiosis by nitrogen starvation, harvested 18 h later and fixed with 70% ethanol for staining with Hoechst33342. At least 200 cells were counted for a mutant. (B) Frequency of abnormal ascospores carrying unequally segregated nuclei in the mcp7Δ, meu13Δ and rad17Δ single and double mutants. Data shown are the average values calculated from at least three independent counting. At least 200 cells were counted for a mutant. (C) Spore viability of the mcp7Δ, meu13Δ and rad17Δ single and double mutants. Random spore analysis was performed. Data shown are the average values calculated from at least three independent assays (84 spores were counted per assay). SDs as error bars are indicated.