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. 2004 Jun 24;32(11):3383–3391. doi: 10.1093/nar/gkh661

Figure 3.

Figure 3

Telomere length measurement from five worms or a single worm by STELA. (A,B) DNA was extracted from five-worm samples independently. After ligation to the telorette, DNA was pooled, and DNA from an equivalent of 0.8 worm, 0.25 worm or 0.08 worm was amplified. Assuming each adult worm contains ∼400 pg of genomic DNA [from 959 somatic cells and ∼1000 germline nuclei per gonad arm; (22,23)], they would represent ∼320, ∼100 and ∼30 pg DNA, respectively, if DNA extraction was 100% efficient. The marker lane is shown on the left and the corresponding telomere length is shown on the right. The blot was hybridized to (A) the subtelomeric probe, or (B) the telomeric probe. (C,D) A single worm is sufficient for telomere length measurement by STELA. DNA was extracted from a single adult worm. After ligation to the telorette, DNA from an equivalent of 0.2 worm was amplified. Assuming each adult worm contains ∼400 pg genomic DNA, it would represent 80 pg of DNA if DNA extraction was 100% efficient. The marker lane is shown on the left and the corresponding telomere length is shown on the right. The blot was hybridized to (C) the subtelomeric probe, or (D) the telomeric probe.