FIG. 4.

Changes in MMP14 and MMP16 protein levels in macaque granulosa cells during the periovulatory period. Monkey granulosa cells were obtained 0, 12, 24, and 36 h after hCG administration from monkeys undergoing controlled ovarian stimulation and processed for protein analysis. A representative immunoblot of MMP14 and MMP16 along with pan-actin (A) in granulosa cell lysates at different times after hCG administration is shown. Densitometry was used to semiquantitate changes in pro-MMP14 and active MMP14 (B) as well as pro-MMP16 and active MMP16 (C) protein in granulosa cell lysates. Arbitrary optical density units measured for proform and active form were normalized to those measured for the internal control actin. The graph depicts the mean ± SEM (n = 4 animals/group). Groups with no common letters are significantly different (P < 0.05).