Figure 2.

Localization of IL-1α in lungs from neonatal wild-type (WT) and Scnn1b-Tg mice. (A) In situ hybridization of lung sections from neonatal (5 d old) WT and Scnn1b-Tg mice with locked nucleic acid–modified sense and antisense IL-1α mRNA detection probes. Representative localization of IL-1α transcripts (dark blue) in airway and lung parenchymal cells in WT and Scnn1b-Tg lungs hybridized with antisense probe. Staining was not detected in lung sections hybridized with sense probes. Scale bars = 100 μm (top) and 10 μm (bottom). (B) Immunohistochemical detection of IL-1α in lung sections from neonatal WT and Scnn1b-Tg mice. Representative localization of IL-1α (brown) in cytoplasm and nuclei of airway epithelial cells and some parenchymal cells in WT and Scnn1b-Tg lungs stained with primary anti–IL-1α antibody (IL-1α). Staining was not detected in lung sections incubated with secondary antibody only (control). All sections were counterstained with hematoxylin. Scale bars = 100 μm (top) and 10 μm (bottom). Representative of n = 3–4 mice per group from six litters.