Table 1. Chemical components and pH of each buffer used in BSA and Hb feedings.
Phosphate buffered saline (PBS), Aedes physiological saline (APS), Sodium phosphate buffered saline (NaPBS), and potassium phosphate buffered saline (KPBS). All concentrations are in mM.
| PBS | APS | NaPBS | KPBS | |
|---|---|---|---|---|
| Sodium Chloride (NaCl) | 136.9 | 150.0 | 137.0 | 2.7 |
| Potassium Chloride (KCl) | 2.7 | 4.0 | 2.7 | 137.0 |
| Disodium Phosphate (Na2HPO4) | 8.1 | 0.0 | 10.0 | 2.0 |
| Sodium Bicarbonate (NaHCO3) | 0.0 | 0.1 | 0.0 | 0.0 |
| Magnesium Chloride (MgCl2) | 0.0 | 0.6 | 0.0 | 0.0 |
| Potassium Dihydrogen Phosphate (KH2PO4) | 1.5 | 0.0 | 2.0 | 10.0 |
| Calcium Chloride (CaCl2) | 0.0 | 1.7 | 0.0 | 0.0 |
| HEPES buffer | 0.0 | 25.0 | 0.0 | 0.0 |
| pH | 7.4 | 7.0 | 7.5 | 7.5 |