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Schematic representation of the AAV2/8(Y733F)-CMV-vector. A pZac2.1 vector plasmid displaying the complementary DNA (cDNA) of human MFRP. (a), human CTRP5 (b), and mouse Mfrp (c) fragments driven by the CMV promoter. The Simian virus 40 (SV40) polyadenylation signal is located at the 3′ end of the cDNA. Arrows indicate the direction of transcription, and the 5′- and 3′-inverted terminal repeat (ITRs) of AAV are shown.
