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. 2014 Jul 8;22(9):1688–1697. doi: 10.1038/mt.2014.100

Figure 4.

Figure 4

Immunoblots and immunofluorescence on MFRP-deficient and CTRP5-overexpressed RPE cells. (a) Antibodies to MFRP, CTRP5, β-actin, CRALBP (RPE-specific marker), and GAPDH were used to probe extracts from iPS-RPE from P1, P2 and the wild-type control donor (lanes 1-3), autopsy RPE without and with AAV-CTRP5-treated (lanes 4-5), and HEK293 cells transduced with AAV-MFRP (lane 6). (b) Immunofluorescence for rhodamine-phalloidin on wild-type iPS-RPE cells (upper left), MFRP-deficient iPS-RPE cells from P1 (upper right), human autopsy RPE (bottom left) and human autopsy RPE treated with AAV-CTRP5 vector (bottom right). Scale bar = 10 μm.