Figure 2.

CCN1 protein increases migration of macrophages and monocytes. Quantification of migrated RAW264.7 macrophages (a) and THP-1 monocytic cells (b) in response to purified CCN1 was determined using a modified Boyden Transwell assay coated with purified CCN1 or BSA. Serum starved RAW264.7 or THP-1 cells were plated in the upper chamber of transwells and allowed to migrate for 6 hours. Following this migration period, nonadherent THP-1 cells which migrated through were counted by hemocytometer while adherent macrophages which migrated through were counted following the fixing and staining of transwell membranes. Data shown are mean number of migrated cells from three independent transwells, quantified as average number of migrated THP-1 cells as determined by hemocytometer and average number of migrated cells/20× view field for RAW264.7.