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. 2014 Jul 29;22(9):1580–1592. doi: 10.1038/mt.2014.112

Figure 1.

Figure 1

Pig CSF1-Fc produces viable CSF1-dependent proliferation in vitro and has extended plasma half-life in vivo. (a) CSF1-Fc molecule was produced by CSF1 joined to the hinge-CH3 region of pig IgG1a. (b) CSF1-dependent Ba/F3pCSF1R cells were cultured in rh-CSF1, harvested, washed twice in PBS, and plated for the optimized cell viability assay with either pig CSF1 or pig CSF1-Fc for 48 hours. Pig BM cells were flushed from an adult pig rib and placed in culture with either pig CSF1 or pig CSF1-Fc for 48 hours. Following addition of MTT solution and solubilization, optical density was read at 570nm using a plate reader. Results are the average of triplicate determinations ± SEM from three experiments. (c) Three weaner pigs were injected with either 0.5 mg/kg or 1.2 mg/kg pig CSF1 or Fc CSF1-Fc respectively and blood collected at time points above for CSF1 and Fc CSF1-Fc levels to be determined by ELISA. The mean ± SEM is graphed.