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. Author manuscript; available in PMC: 2015 May 18.
Published in final edited form as: Dev Biol. 2011 Jul 1;357(1):152–164. doi: 10.1016/j.ydbio.2011.06.041

Figure 9. Genetic reduction of versican in Adamts5−/− mice significantly rescues the phenotype of developing pulmonary valves.

Figure 9

Frontal sections of E17.5 hearts from the indicated genotypes were immunostained for intact versican (GAGβ - green; propidium iodide - red; α-sarcomeric actin - blue) (A, B, C, D) or with H & E to outline their morphology (E, F, G, H). Three-dimensional reconstructions of the PV from Adamts5+/+; Vcan+/+ (I); Adamts5−/−; Vcan+/+ (J) and Adamts5−/−; Vcan+/− mice (K) were generated using Amira™ reconstruction software to outline their shape, and obtain volume measurements of the PV at E17.5. Reconstruction volumes listed (Supplemental Table 1, also includes aortic valve data). Sections of PV cusps of Adamts5+/+,Vcan+/+ (M); Adamts5−/−; Vcan+/+ (N) or Adamts5−/−; Vcan+/− mice (O) were H & E stained. Mesenchymal cell compaction (cells/unit area) was measured using Amira™ software (L) and is expressed as mean ±SD of the mean. * denotes a significant difference in the compaction of the Adamts5−/−; Vcan+/− PV compared to Adamts5−/−; Vcan+/+ mice (P<0.003) and of Adamts5−/−; Vcan+/− PV compared to Adamts5+/+; Vcan+/+ PV (P<0.008). BMP2 immunolocalization (P, Q, R) of the indicated genotypes. Sox9 localization depicted in panels S, T, U. PA- pulmonary artery; RV-right ventricle; Rt-right cusp of the PV; An-anterior cusp of the PV; Lt-left cusp of the PV. Scale bars: A = 150 μm, E, P, S = 100 μm; I = 200 μm; M = 30 μm.