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. 2014 May 27;22(8):1518–1529. doi: 10.1038/mt.2014.61

Figure 2.

Figure 2

Expanded podoplanin-positive monocytes (PPMs) express lymphangiogenesis-related genes. (a) Protein expression of podoplanin, VEGFR-3, and LYVE-1 in hLEC, fresh PBMCs, and cultured hematospheres at days 3 and 5 via western blot analysis. PPMs also expressed VEGFR-3 and LYVE-1. β Actin was utilized as an internal control. (b) Whole-mount immunofluorescence staining of the cultured hematospheres on day 5 with podoplanin and VEGFR-3. Scale bar = 50 µm. (c) Gene expression of lymphangiogenesis-related genes in fresh PBMCs and cultured hematospheres at days 3 and 5 via semiquantitative RT-PCR. PPMs showed a significant induction of lymphatic endothelial cell–specific genes. GAPDH was utilized as an internal control. Ang, angiopoietin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; hLEC, human lymphatic endothelial cell; PBMCs, peripheral blood mononuclear cells; TGF, transforming growth factor; VEGF, vascular endothelial growth factor; VEGFR, vascular endothelial growth factor receptor.