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. Author manuscript; available in PMC: 2016 Apr 30.
Published in final edited form as: Cell Physiol Biochem. 2015 Apr 30;36(1):208–220. doi: 10.1159/000374065

Fig. 4.

Fig. 4

Protein palmitoylation is necessary for activation of Nox2 and ROS generation. Endothelial cells, incubated in normal or high glucose for 3 hours or 96 hours in the absence or presence of 2-bromopalmitate (2-BP), were analyzed for Nox2 activity (Panels A & B respectively), and for quantification of ROS levels using DCHFDA (Panels C & D respectively). 5mM and 20mM=cells incubated in 5mM glucose or 20mM glucose, respectively and Mann= cells incubated in mannitol [as an osmotic control]. *P < 0.001 vs. 5mM glucose and **P < 0.001 vs. 20mM glucose.