Skip to main content
. 2015 May 13;6:7029. doi: 10.1038/ncomms8029

Figure 2. PalmtdTomato labels different-sized EVs and co-labels with PKH.

Figure 2

(a) Dot blot detection of PalmtdTomato+ EV isolated from 0.22 or 0.8 μm filtered conditioned medium of 293T-PalmtdTomato cells. (b) Confocal microscopy of EVs isolated from 0.22 or 0.8 μm filtered conditioned medium of 293T-PalmtdTomato cells. Scale bar, 10 μm. (c) Digital display of PalmtdTomato+ EVs signals as spheres. Scale bar, 10 μm. (d) Quantification of PalmtdTomato+ EV signals per field in 0.22 or 0.8 μm filtered samples. *P<0.001 by two-tailed, t-test with 14 replicates. (e) Nanoparticle tracking analysis (NTA) of PalmtdTomato+ EVs isolated from 0.22 and 0.8 μm filtered samples. *P<0.05 by two-tailed t-test with six replicates. The results are presented as the mean ± s.e.m. (f) EV-depleted medium (top rows) or EVs isolated from 293T-PalmtdTomato cells (bottom rows) were stained with PKH67 followed by a 1 h wash in PBS at 100,000 g. Scale bar, 20 μm. (g,h) Semi-quantification of EV signals from PKH67-stained EV-depleted medium (g) or PalmtdTomato+ EVs co-labelling with PKH67 (h). *P<0.05 by repeated measures analysis of variance (ANOVA) followed by Tukey's post hoc test with four replicates. The results are presented as the mean ± s.e.m.