Table 2.
Process relevant data from batch cultures
| Medium | ActiCHO P | CD CHO | CD OptiCHO | Ex-Cell CD CHO | ProCHO5 | BalanCD | Cellvento CHO-100 | CD FortiCHO |
|---|---|---|---|---|---|---|---|---|
| Peak cell conc. (106 cells/mL) | 8.5 | 2.5 | 3.9 | 2.6 | 4.5 | 9.0 | 4.9 | 0.6 |
| Antibody conc. (mg/L) | 863 | 334 | 522 | 510 | 384 | 778 | 503 | n.d. |
| μ exponential phase (1/d) | 0.70 | 0.52 | 0.41 | 0.42 | 0.47 | 0.49 | 0.41 | 0.31 |
| qP exp. phase (pg/(cell × day)) | 49.6 | 71.8 | 49.1 | 44.6 | 53.4 | 44.4 | 30.4 | n.d. |
| qP whole process (pg/(cell × day)) | 41.9 | 42.7 | 35.2 | 36.8 | 49.9 | 29.3 | 28.5 | n.d. |
| STY (mg/(L × d)) | 121 | 28 | 46 | 44 | 51 | 76 | 45 | n.d. |
| qGlucose (pg/(cell × day)) | −368 | −482 | −446 | −434 | −319 | −149 | −237 | −647 |
| qLactate (pg/(cell × day)) | 142 | 421 | 221 | 168 | 296 | 103 | 93 | 485 |
| qGlutamate (pg/(cell × day)) | −19 | −6 | −13 | −2 | 8 | 22 | −3 | 3 |
| qGlutamine (pg/(cell × day)) | −83 | −85 | −89 | −80 | −143 | −72 | −82 | −99 |
| qAmmonium (pg/(cell × day)) | 7 | 20 | 10 | 13 | 16 | 12 | 11 | 21 |
All values have been calculated as mean
STY space-time yield, n.d. not determined