Table 2.
Optimized combination of 12 commercially available flourochrome-conjugated antibodies to reliably quantify 14 subpopulations of immune cells
| Conjugation | Name | Company (clone) |
|---|---|---|
| FITC | Anti-human CD56 antibody | BD (clone: MEM188) |
| PE | Anti-human SCF R/c-kit antibody | R&D (clone: 47233) |
| PerCP-Cy5.5 | Anti-human CD123 antibody | eBioscience (clone: 7G3) |
| PE-Cy7 | Anti-human CD11c antibody | eBioscience (clone: 3.9) |
| V450 | Anti-human CD45 antibody | BD (clone: HI30) |
| AmCyan | Anti-human CD8 antibody | BD (clone: SK1) |
| eFluor 605 Nanocrystal | Anti-human CD19 antibody | eBioscience (clone: HIB19) |
| eFluor 655 Nanocrystal | Anti-human CD3 antibody | eBioscience (clone: OKT3) |
| Qdot 705 | Anti-human CD4 antibody | Invitrogen (clone: S3.5) |
| APC | Anti-human TCRγδ antibody | BD (clone: B1) |
| Alexa Fluor 700 | Anti-human CD14 antibody | BioLegend (clone: HCD14) |
| APC-Cy7 | Anti-human HLA-DR antibody | eBioscience (clone: LN3) |