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. Author manuscript; available in PMC: 2015 May 18.
Published in final edited form as: J Biomol Screen. 2012 Dec 10;18(6):647–658. doi: 10.1177/1087057112469405

Figure 3.

Figure 3

Hit compounds alter the secretion of untagged fibulin-3. (A) ARPE19 cells were infected with adenovirus encoding for β-galactosidase (LacZ), untagged wild-type (WT) fibulin-3, untagged R345W fibulin-3, or untagged WT fibulin-5 (an approximate multiplicity of infection [MOI] of 50) for 48 h followed by 24 h treatment with the indicated compound (10 μM). Portions of the conditioned media after drug treatment were analyzed by Western blotting and probed with anti–fibulin-3 (FBLN3), anti-ERdj3, or anti–fibulin-5 (FBLN5) antibodies (representative data of three independent experiments). Endogenous ERdj3 was monitored in cells infected with WT fibulin-3 at an MOI of 50. (B) LI-COR quantification of the band intensity of conditioned media samples (n ≥ 3, ± SEM).