Skip to main content
. Author manuscript; available in PMC: 2015 Nov 13.
Published in final edited form as: Nat Commun. 2015 May 13;6:7114. doi: 10.1038/ncomms8114

Figure 6. IFNγ from endogenous CD8 T-cells controls CCL21 but not PNAd expression in i.p. tumors.

Figure 6

(a–d) Infiltration of naïve OT-I cells into i.p. (a,b,d) or s.c. (c) B16-OVA tumors in the indicated mice was determined as in Figure 2. n=7 (a), 3 (b,d), 5 (c). In (d), Rag2−/− mice were repleted with either WT or IFNγ−/− CD8 T-cells prior to tumor implantation.

(e) The absolute number of CD8+ T-cells (non-OT-I) that had accumulated in i.p. B16-OVA tumors in mice with the indicated background was determined by flow cytometry. n=3 per group.

(f,g) PNAd expression in i.p. tumors from the indicated mice was quantified as in Figure 3. n=3 per group.

(h–j) Ccl21 (h,j) or Ccl19 (i) expression in i.p. tumors lysates from the indicated mice was quantified. Ccl21 data (h,j) is presented as 2^−ΔΔCT method relative to HPRT with expression levels in control samples normalized to 1. Ccl19 data (i) is presented as 2^−ΔCT relative to HPRT. Dashed line represents Ccl21 expression levels in control tumors. n=3–6 per group.

Data (mean+SEM) are representative at least two independent experiments. ns:p>0.05, *p<0.05, **p<0.01, ***p<0.001 by unpaired t-test (a-c,f-h) or one way ANOVA with Tukey’s post-test (d,i).