Fig. 6.
Effect of Ang II on intracellular free calcium in cultured rat cardiomyocytes. (A) Effect of Ang II (10 nmol/L). (B) Effect of the selective adenosine A1 agonist CCPA (1 μmol/L) on the change in intracellular free calcium induced by Ang II (10 nmol/L). (C) Effect of the adenosine A3 agonist IB-MECA (0.1 μmol/L) on the change in intracellular free calcium induced by Ang II (10 nmol/L). (D) Effect of Ang II (10 nmol/L) in a calcium free buffer. Cultures were incubated with agonists for 5 min at room temperature prior to the addition of Ang II. Similar results were obtained in 5 different experiments.