Fig. 3.

a Western blot analysis showed the efficiency of Ubc9-lentivirus and Ubc9 siRNA on the expression of Ubc9 in differentiated SH-SY5Y cells. Sham (cells without treatment); siRNA-c (cells treated with Allstars Negative Control siRNA); Ubc9 siRNA (cells treated with Ubc9 siRNA); lenti-c (cells treated with control lentivirus); Ubc9 lenti (cells treated with Ubc9 lentivirus). Data are presented as mean ± SEM. **p < 0.01 compared with the sham group. b Histograms showing the percentage of cell viabilities in Ubc9 siRNA or Ubc9 lentivirus-transfected cells and non-transfected cells when subjected to different treatments. Sham (without treatment), Iso (isoflurane only), OGD (oxygen-glucose deprivation), IsoPC (isoflurane preconditioning plus OGD), siRNA + OGD (Ubc9 siRNA plus OGD), siRNA + IsoPC (Ubc9 siRNA plus isoflurane preconditioning plus OGD), lenti + OGD (Ubc9 lentivirus plus OGD), lenti + IsoPC (Ubc-lentivirus plus isoflurane preconditioning plus OGD). Data are presented as mean ± SEM. *p < 0.05 compared with the sham group. #p < 0.05 compared with IsoPC group