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. Author manuscript; available in PMC: 2015 May 18.
Published in final edited form as: Curr Biol. 2010 Jul 22;20(16):1470–1475. doi: 10.1016/j.cub.2010.06.072

Figure 1. Two-Photon Calcium Imaging of the Motion-Sensitive HSN Neuron in Drosophila.

Figure 1

(A) Schematic of the walking fly recording setup.

(B) We recorded from the HSN’s dendrite branch, outlined in dotted red (from fly 6). HSE: HS-Equatorial. Scale bars represent 20 µm (i) and 5 µm (ii).

(C) Canonical responses of the HSN neuron (from fly 6) to a horizontally rotating vertical grating pattern moving at a temporal frequency of 1 Hz in trials in which the fly was standing still. We always recorded from the left half of the brain; therefore, a front-to-back preferred direction (PD) for the neuron corresponds to counterclockwise rotations of the pattern.

(D) Temporal frequency tuning of HSN dendrites. Shown are the mean normalized peak responses across eight different flies. Error bars indicate mean ± standard error of the mean (SEM).

(E) Directional tuning of HSN dendrites. The figure shows the mean normalized peak response (error bars indicate mean ± SEM) across four different flies, during 10 s of grating motion of different orientations (indicated by red arrows) moving at 1 Hz. In all cases, the peak response was calculated as the mean value from a 0.5 s time window centered at the peak of the response and was normalized to the neuron’s maximum peak response.