Table II.
HIV-1 replication and MCHI induction on DP thymocytes in HF-TOC
| HIV-1 (N)a | Replicationb | Coreceptor Usagec | Fold Induction (SE)d |
|---|---|---|---|
| NL4-3 (11) | + | CXCR4 | 9 (2.6) |
| JD (5) | + | CXCR4/CCR5 | 19 (5.7) |
| EW (4) | + | CXCR4 | 24 (4.6) |
| HXB2 (11) | − | CXCR4 | 1.2 (0.6) |
| 89.6 | + | CXCR4/CCR5 | 7 (1.9) |
| JRCSF | + | CCR5 | 5 (1.7) |
a
HIV-1 clones/isolates with different replication (p24 production) were compared in MHCI induction. N is the number of HF-TOC assays analyzed. HXB2 is unable to replicate in HF-TOC or SCID-hu Thy/Liv mice (23).
b
Peak p24 levels of >5000 pg/106 thymocytes are indicated as +; levels of <50 pg/106 thymocytes are indicated as −.
c
HIV-1 coreceptor usage was determined by CXCR4- and CCR5-dependent fusion or replication assays. JD and EW were tested for this study (K.D. and L.S., unpublished results).
d
Fold induction indicates mean channel fluorescence (MCF) of MHCI (W6/32) staining on DP thymocytes from HIV-infected HF-TOC divided by MCF on DP thymocytes from mock-infected HF-TOC.