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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Ann Biomed Eng. 2013 Oct 17;42(7):1357–1372. doi: 10.1007/s10439-013-0921-4

Table 3.

hPSC aggregate-based suspension culture systems in spinner flasks and their clinical compliancea

References Media Volume
(ml)		 Cell line
tested (#)		 Passage Folds
(days)		 Max yield
(cells ml−1)		 Characterization Undifferentiated (%)b XF CD Eco
Krawetz et al.51 mTeSR1 100 ES (1) 4 5 (5–6) 1.6 × 106 Full 76 – 98 ○ ○ ○
Singh et al.99 mTeSR1 50 ES (3) 5 2 (7) 2–2.4 × 106 Full 85 – 98 ○ ○ ○
Zweigerdt et al.135 mTeSR1 50 ES (3)
iPS (2)		 10 2–3 (4–7) 2–3 × 106 Full > 85 ○ ○ ○
Amit et al.4 IL6RIL6c 50 ES (7)
iPS (6)		 20 17.7 (6) 1.89 × 106 Full ND ○ ○ ○
Abbasalizadeh et al.1 HFF-CMd 100 ES (2)
iPS (2)		 20* 8 (7–10) 2.8 × 106 Partial 95 – 98 ● ○ ○
Olmer et al.83 mTeSR1 100 iPS (1) 2* 5.5 (7) 2 × 106 Partial 69 – 97 ○ ○ ○
Chen et al.17 StemPro 60–250 ES (3) 21 3.2–4.3 (3–4) 1.1 × 106 Full > 90 ○ ○ ○
Wang et al.119 E8 50 iPS (2) 20 2.8–3.5 (3) 1.8 × 106 Full 95 – 99 ● ● ●
a

Meaning of items: see Table 1.

b

Undifferentiated: undifferentiated population that was positively stained with markers indicating undifferentiated stage of hPSCs, including TRA-1-60, TRA-1-81, SSEA-3, SSEA-4, Oct-4, Nanog, Sox2, etc.

c

IL6RIL6: DMEM/F12 medium supplemented with KO-serum replacement, nonessential amino acid (NEAA), L-glutamine, β-mercaptoethanol, rh bFGF, and rh full-length interleukin-6 and interleukin-6 receptor (IL6RIL6) chimera.

d

HFF-CM: Human foreskin fibroblasts conditioned DMEM/F12, GlutaMAX, rh bFGF.

*

Karyotype was not determined.

Abbreviations: XF: xeno-free; CD: chemically defined; Eco: economically efficient; ND: not determined.