Table 3.
References | Media | Volume (ml) |
Cell line tested (#) |
Passage | Folds (days) |
Max yield (cells ml−1) |
Characterization | Undifferentiated (%)b | XF | CD | Eco |
---|---|---|---|---|---|---|---|---|---|---|---|
Krawetz et al.51 | mTeSR1 | 100 | ES (1) | 4 | 5 (5–6) | 1.6 × 106 | Full | 76 – 98 | ○ | ○ | ○ |
Singh et al.99 | mTeSR1 | 50 | ES (3) | 5 | 2 (7) | 2–2.4 × 106 | Full | 85 – 98 | ○ | ○ | ○ |
Zweigerdt et al.135 | mTeSR1 | 50 | ES (3) iPS (2) |
10 | 2–3 (4–7) | 2–3 × 106 | Full | > 85 | ○ | ○ | ○ |
Amit et al.4 | IL6RIL6c | 50 | ES (7) iPS (6) |
20 | 17.7 (6) | 1.89 × 106 | Full | ND | ○ | ○ | ○ |
Abbasalizadeh et al.1 | HFF-CMd | 100 | ES (2) iPS (2) |
20* | 8 (7–10) | 2.8 × 106 | Partial | 95 – 98 | ● | ○ | ○ |
Olmer et al.83 | mTeSR1 | 100 | iPS (1) | 2* | 5.5 (7) | 2 × 106 | Partial | 69 – 97 | ○ | ○ | ○ |
Chen et al.17 | StemPro | 60–250 | ES (3) | 21 | 3.2–4.3 (3–4) | 1.1 × 106 | Full | > 90 | ○ | ○ | ○ |
Wang et al.119 | E8 | 50 | iPS (2) | 20 | 2.8–3.5 (3) | 1.8 × 106 | Full | 95 – 99 | ● | ● | ● |
Meaning of items: see Table 1.
Undifferentiated: undifferentiated population that was positively stained with markers indicating undifferentiated stage of hPSCs, including TRA-1-60, TRA-1-81, SSEA-3, SSEA-4, Oct-4, Nanog, Sox2, etc.
IL6RIL6: DMEM/F12 medium supplemented with KO-serum replacement, nonessential amino acid (NEAA), L-glutamine, β-mercaptoethanol, rh bFGF, and rh full-length interleukin-6 and interleukin-6 receptor (IL6RIL6) chimera.
HFF-CM: Human foreskin fibroblasts conditioned DMEM/F12, GlutaMAX, rh bFGF.
Karyotype was not determined.
Abbreviations: XF: xeno-free; CD: chemically defined; Eco: economically efficient; ND: not determined.