FIG 5 .

LegK2 inhibits late endosome trafficking to LCVs. (A) Acquisition of vacuolar proton ATPase on LCVs. D. discoideum was infected for 1 h at an MOI of 100 with mCherry-labeled legK2 mutant L. pneumophila. The presence of vacuolar H⁺-ATPase on LCVs was detected by an immunofluorescence assay with anti-VatA antibodies. The inset shows typical VatA-positive vacuoles. (B) Acquisition of vacuolar proton ATPase in legK2 mutant-containing vacuoles. VatA-positive vacuoles (n = >100) in amoebae infected with WT L. pneumophila Lens, the derivative dotA and legK2 mutants, and the transformed legK2(plegK2) and legK2(plegK2cat) mutants were counted. These data are representative of three independent experiments, and the error bars represent the standard deviations. **, P < 0.01.