FIG 4 .
Effects of CsA on SHP signaling and biofilm formation in different S. pyogenes strains. (A) Relative luminescence activity of the Pshp2-luxAB reporter in response to the addition of 10 nM SHP2-C8 pheromone and 5 µM CsA. Bars show standard deviations. (B) S. pyogenes strains grown in 24-well plates were treated with 10 nM SHP2-C8 and 5 µM CsA. At 20 h, biofilms were assessed by a standard crystal violet staining method. Error bars indicate standard errors from a minimum of three independent experiments. (C) Picture of one representative biofilm experiment.