TABLE 3 .
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference |
|---|---|---|
| Strains | ||
| NZ131 | Wild-type S. pyogenes M49 strain | 64, 65 |
| BNL148 | NZ131 integrated with pBL111 Pshp2-luxAB reporter; Ermr | 20] |
| BNL178 | NZ131 Δrgg3 shp3GGG shp2GGG::Pshp2-luxAB | 21 |
| MGAS315(pWAR200) | S. pyogenes M3 isolate with PsigX-luxAB reporter on plasmid pWAR200 | 37, 66 |
| UA159(pWAR304) | S. mutans isolate with PsigX-luxAB on plasmid pWAR304 | 35, 67 |
| NZ131(pWAR193) | S. pyogenes M49 strain with PspeB-luxAB reporter on plasmid pWAR193; Ermr | This study |
| A909(pSar110) | Wild-type S. agalactiae A909 clinical isolate with Pshp1520-luxAB cloned in pLZ12-spec | 33, 68, 69 |
| GGS-LT1(pLC301) | S. dysgalactiae subsp. equisimilis strain with Pshp2-luxAB on pLZ12-spec | 33; this study |
| NCTC 10999(pJC254) | Wild-type S. porcinus Collins et al. (ATCC 43138) strain with Pshp3-luxAB reporter on pJC254; Specr | 70; this study |
| Plasmids | ||
| p7INT | Shuttle-suicide vector that integrates at streptococcal bacteriophage T12 attB site; Ermr | 71 |
| pLZ12-spec | Shuttle vector encoding spectinomycin resistance; pWV01 origin; Specr | 60 |
| pJC254 | 227-bp upstream region of shp3 fused to luxAB genes and cloned in BamHI and EcoRI sites of pLZ12-spec | This study |
| pWAR368 | pET 15b expression vector with UA159 comR cloned into NdeI and BamHI restriction sites; Ampr | This study |
| pWAR193 | 947-bp upstream region of NZ131 speB cloned in front of luxAB in p7INT; Ermr | This study |
| pLC301 | 500-bp upstream region of S. pyogenes shp2 fused to luxAB genes and cloned in pLZ12-spec; Specr | This study |