TABLE 1 .
Composition and molecular mass analyses of alginate produced by different mutantsa
| No. | Mutant description | FG | FM | FGM/MG | FMM | Ac. % | Wt avg molecular mass (kDa) | No. avg molecular mass (kDa) | PI | Alginate yield (g)/CDM (g) |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 300+MCS5 | 0.3 | 0.7 | 0.29 | 0.41 | 32 | 3,927 (±0.864%) | 3,832 (±0.842%) | 1.025 (±1.2%) | 1.3 ± 0.03 |
| 2 | 300Δ8+MCS5:alg8 | 0.18 | 0.82 | 0.17 | 0.65 | 11.3 | 3,045 (±0.556%) | 3,037 (±0.551%) | 1.003 (±0.7%) | 12.8 ± 1.03 |
| 3 | 300Δ44+MCS5:alg44 | 0.18 | 0.82 | 0.18 | 0.64 | 26.8 | 3,831 (±0.963%) | 3,650 (±0.950%) | 1.05 (±1.3%) | 8.7 ± 0.53 |
| 4 | 300Δ44Δ8+MCS5:alg44:alg8 | 0.17 | 0.83 | 0.17 | 0.66 | 11 | 3,369 (±0.839%) | 3,352 (±0.821%) | 1.005 (±1.1%) | 41.5 ± 4.9 |
| 5 | 300ΔX+MCS5:algX | 0.36 | 0.64 | 0.36 | 0.28 | 9.8 | 2,460 (±0.932%) | 2,447 (±0.913%) | 1.005 (±1.3%) | 104.1 ± 5.5 |
| 6 | 300ΔX+MCS5:algX(S269A) | 0.36 | 0.64 | 0.36 | 0.28 | 0 | 2,086 (±0.960%) | 2,065 (±0.944%) | 1.010 (±1.3%) | 125.8 ± 9.9 |
| 7 | 300ΔG+MCS5:algG | 0.32 | 0.68 | 0.32 | 0.36 | 23.3 | 2,755 (±1.041%) | 2,726 (±0.986%) | 1.011 (±1.4%) | 2.6 ± 0.04 |
| 8 | 300ΔG+MCS5:algG(D324A) | 0 | 1 | 0 | 1 | 25.2 | 4,653 (±1.097%) | 4,575 (±1.117%) | 1.017 (±1.5%) | 7.6 ± 0.57 |
| 9 | 300ΔXΔG+MCS5:algX:algG | 0.34 | 0.66 | 0.34 | 0.32 | 28.4 | 3,076 (±1.051%) | 3,044 (±1.029%) | 1.011 (±1.4%) | 67.42 ± 4.8 |
| 10 | 300ΔXΔG+MCS5:algX(S269A):algG(D324A) | 0 | 1 | 0 | 1 | 0 | 1,811 (±0.884%) | 1,716 (±0.888%) | 1.055 (±1.2%) | 8.7 ± 0.42 |
| 11 | 300Δ44ΔG+MCS5:alg44+algG | 0.22 | 0.78 | 0.22 | 0.56 | 14.5 | 2,907 (±0.966%) | 2,861 (±0.944%) | 1.016 (±1.3%) | 9.0 ± 0.3 |
a
300, PDO300; MCS5, pBBR1MCS-5; FG, molar fraction of guluronate (G) residue; FM, molar fraction of mannuronate (M) residue; FGM/MG, molar fraction of two consecutive G and M residues; FMM, molar fraction of two consecutive M residues; Ac, acetylation; PI, polydispersity index; CDM, cell dry mass.