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. 2015 May 14;8:272. doi: 10.1186/s13071-015-0874-6

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© Satho et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Oviposition bioassay design. The cups were placed on the dish, which was positioned at the bottom center of the cage. Cups were placed such that each was at an identical distance from the adjacent cup. A: The four oviposition cups contained one of the following media: (i) HCE, (ii) MCE, (iii) cooled boiled water (WAa), or (iv) cooled boiled water (WAb); B: The four oviposition cups contained one of the following media: (i) HCE, (ii) cooled boiled water (WAa), (iii) cooled boiled water (WAb), or (iv) cooled boiled water (WAc); C: The four oviposition cups contained one of the following media: (i) HCEa, (ii) HCEb, (iii) HCEc, and (iv) cooled boiled water (WA).