Fig 5. PRDX1 is not released associated with influenza virions.

A549 cells were infected (+) or mock infected (-) as described in the Methods. Supernatants were blocked with 40 mM NEM, concentrated and the virions immunoprecipitated with anti-influenza antibody. Immunoprecipitated samples and supernatants left after immunoprecipitation were run under non-reducing (A) or reducing conditions (B) and analyzed by Western blot with anti-PRDX1 (A) or anti-HA (B) antibody.