Table 1. Comparison of some important DAPs in seedling leaves of NC47 and CS.
Spot no. | Protein name | Accession no. | Predicted subcellular localisation a | Average volume ratio CS0:15%:20%:25%:30% | Average volume ratio NC470:15%:20%:25%:30% |
---|---|---|---|---|---|
Amino acid metabolism | |||||
5 | Methionine synthase 1 enzyme | gi|68655495 | Cyto | 1: 0.69: 1.02: 0.00: 0.00 | 1: 0.60: 0.69: 0.26: 0.13 |
Carbon metabolism | |||||
7 | Cytosolic aconitase | gi|290783890 | Cyto | 1: 1.51: 3.94: 1.97: 2.76 | 1: 0.80: 1.51: 1.04: 1.76 |
16 | Phosphoglycerate mutase | gi|32400802 | Cyto | 1: 1.36: 0.90: 0.65: 0.74 | 1: 1.52: 2.05: 1.09: 1.97 |
17 | NADP-dependent malic enzyme | gi|158701881 | P | 1: 1.12: 1.03: 1.83: 0.88 | 1: 1.70: 1.68: 2.43: 2.31 |
19 | NADP-dependent malic enzyme | gi|158701881 | P | 1: 2.41: 3.34: 3.04: 1.98 | 1: 0.97: 2.35: 2.38: 3.14 |
22 | Enolase | gi|90110845 | Cyto | N b | 1: 1.30: 2.04: 1.83: 2.33 |
26 | 6-phosphogluconate dehydrogenase, decarboxylating | gi|357110692 | Mito | N | 1: 1.77: 1.22: 1.51: 4.91 |
40 | Chloroplast fructose-bisphosphate aldolase | gi|223018643 | P | 1: 0.58: 0.60: 0.00: 0.00 | 1: 0.72: 0.59: 0.56: 0.00 |
45 | Glyceraldehyde 3-phosphate dehydrogenase | gi|15222111 | Cyto | 1: 1.06: 0.56: 1.15: 1.21 | 1: 1.00: 0.82: 0.63: 1.79 |
90 | β-amylase | gi|3334120 | P | N | 1: 0.83: 0.86: 0.75: 1.73 |
97 | Triosephosphate-isomerase | gi|11124572 | Cyto | 1:1.44: 1.41: 2.10: 1.36 | 1: 1.19: 1.13: 1.13: 2.58 |
Energy pathway | |||||
14 | vacuolar proton-ATPase subunit A | gi|90025017 | chloroplast | 1: 1.77: 1.28: 0.81: 1.71 | 1: 1.22: 1.39: 1.65: 2.24 |
Nucleotide metabolism | |||||
58 | Chitinase 2 | gi|18146827 | chloroplast | N | 1: 1.23: 1.51: 1.23: 2.01 |
Photosynthesis/ Respiration | |||||
64 | Oxygen-evolving enhancer protein 2 | gi|131394 | P | 1: 1.79: 1.35: 3.23: 3.05 | 1: 0.83: 0.90: 1.53: 3.21 |
65 | Oxygen-evolving enhancer protein 2 | gi|131394 | P | 1: 4.15: 2.79: 6.07: 7.04 | 1: 1.16: 3.45: 4.65: 8.18 |
93 | Phosphoribulokinase | gi|125580 | P | N | 1: 0.21: 0.34: 0.35: 0.29 |
Stress/defence/detoxification | |||||
33 | Dehydroascorbate reductase | gi|28192421 | Mito | 1: 0.20: 0.89: 0.33: 0.17 | 1: 1.24: 0.46: 0.32: 0.74 |
62 | Ascorbate peroxidase | gi|3688398 | Cyto | N | 1: 0.61: 0.42: 0.66: 1.06 |
76 | 2-Cys peroxiredoxin BAS1 | gi|2829687 | P | 1: 1.89: 0.83: 1.23: 1.06 | 1: 1.52: 2.05: 0.97: 2.31 |
95 | Fibrillin-like protein | gi|29367475 | P | N | 1: 1.83: 0.99: 2.03: 1.72 |
Transcription/Translation | |||||
63 | Predicted protein | gi|326490946 | P | 1: 1.08: 0.76: 1.89: 1.37 | 1: 1.25: 1.32: 1.37: 2.67 |
69 | 50S ribosomal protein L1 | gi|195638036 | Nucl | 1: 0.70: 0.53: 1.23: 1.18 | 1: 1.31: 0.90: 0.90: 2.46 |
Protein folding | |||||
3 | 70-kDa heat shock protein | gi|254211611 | P | N | 1: 2.12: 2.70: 2.32: 2.63 |
11 | RuBisCO large subunit-binding protein subunit | gi|2493650 | P | 1: 0.47: 0.57: 0.93: 1.03 | 1: 0.66: 0.89: 0.92: 1.62 |
aCyto, cytoplasm; P: plastid; Mito: mitochondria; Nucl: nuclear; PM: Plasma membrane.
b"N" represent the DAP spot was no obvious difference (< 2.0-fold).