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. 2015 May 18;10(5):e0125302. doi: 10.1371/journal.pone.0125302

Table 1. Comparison of some important DAPs in seedling leaves of NC47 and CS.

Spot no. Protein name Accession no. Predicted subcellular localisation a Average volume ratio CS0:15%:20%:25%:30% Average volume ratio NC470:15%:20%:25%:30%
Amino acid metabolism
5 Methionine synthase 1 enzyme gi|68655495 Cyto 1: 0.69: 1.02: 0.00: 0.00 1: 0.60: 0.69: 0.26: 0.13
Carbon metabolism
7 Cytosolic aconitase gi|290783890 Cyto 1: 1.51: 3.94: 1.97: 2.76 1: 0.80: 1.51: 1.04: 1.76
16 Phosphoglycerate mutase gi|32400802 Cyto 1: 1.36: 0.90: 0.65: 0.74 1: 1.52: 2.05: 1.09: 1.97
17 NADP-dependent malic enzyme gi|158701881 P 1: 1.12: 1.03: 1.83: 0.88 1: 1.70: 1.68: 2.43: 2.31
19 NADP-dependent malic enzyme gi|158701881 P 1: 2.41: 3.34: 3.04: 1.98 1: 0.97: 2.35: 2.38: 3.14
22 Enolase gi|90110845 Cyto N b 1: 1.30: 2.04: 1.83: 2.33
26 6-phosphogluconate dehydrogenase, decarboxylating gi|357110692 Mito N 1: 1.77: 1.22: 1.51: 4.91
40 Chloroplast fructose-bisphosphate aldolase gi|223018643 P 1: 0.58: 0.60: 0.00: 0.00 1: 0.72: 0.59: 0.56: 0.00
45 Glyceraldehyde 3-phosphate dehydrogenase gi|15222111 Cyto 1: 1.06: 0.56: 1.15: 1.21 1: 1.00: 0.82: 0.63: 1.79
90 β-amylase gi|3334120 P N 1: 0.83: 0.86: 0.75: 1.73
97 Triosephosphate-isomerase gi|11124572 Cyto 1:1.44: 1.41: 2.10: 1.36 1: 1.19: 1.13: 1.13: 2.58
Energy pathway
14 vacuolar proton-ATPase subunit A gi|90025017 chloroplast 1: 1.77: 1.28: 0.81: 1.71 1: 1.22: 1.39: 1.65: 2.24
Nucleotide metabolism
58 Chitinase 2 gi|18146827 chloroplast N 1: 1.23: 1.51: 1.23: 2.01
Photosynthesis/ Respiration
64 Oxygen-evolving enhancer protein 2 gi|131394 P 1: 1.79: 1.35: 3.23: 3.05 1: 0.83: 0.90: 1.53: 3.21
65 Oxygen-evolving enhancer protein 2 gi|131394 P 1: 4.15: 2.79: 6.07: 7.04 1: 1.16: 3.45: 4.65: 8.18
93 Phosphoribulokinase gi|125580 P N 1: 0.21: 0.34: 0.35: 0.29
Stress/defence/detoxification
33 Dehydroascorbate reductase gi|28192421 Mito 1: 0.20: 0.89: 0.33: 0.17 1: 1.24: 0.46: 0.32: 0.74
62 Ascorbate peroxidase gi|3688398 Cyto N 1: 0.61: 0.42: 0.66: 1.06
76 2-Cys peroxiredoxin BAS1 gi|2829687 P 1: 1.89: 0.83: 1.23: 1.06 1: 1.52: 2.05: 0.97: 2.31
95 Fibrillin-like protein gi|29367475 P N 1: 1.83: 0.99: 2.03: 1.72
Transcription/Translation
63 Predicted protein gi|326490946 P 1: 1.08: 0.76: 1.89: 1.37 1: 1.25: 1.32: 1.37: 2.67
69 50S ribosomal protein L1 gi|195638036 Nucl 1: 0.70: 0.53: 1.23: 1.18 1: 1.31: 0.90: 0.90: 2.46
Protein folding
3 70-kDa heat shock protein gi|254211611 P N 1: 2.12: 2.70: 2.32: 2.63
11 RuBisCO large subunit-binding protein subunit gi|2493650 P 1: 0.47: 0.57: 0.93: 1.03 1: 0.66: 0.89: 0.92: 1.62

aCyto, cytoplasm; P: plastid; Mito: mitochondria; Nucl: nuclear; PM: Plasma membrane.

b"N" represent the DAP spot was no obvious difference (< 2.0-fold).