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. 2015 May 13;10:3519–3531. doi: 10.2147/IJN.S80502

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© 2015 Liu et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Functionalized self-assembling peptide improved insulin secretion function in INS-1 β-cells.

Notes: (A) Insulin secretion, (B) intracellular insulin (under 20 mM glucose), and (C) GSIS index in the different groups after 3 days of culture. (D) Real-time polymerase chain reaction analysis of Glut2, Ins1, MfaA, and Pdx-1 mRNA expression after 3 days of culture. (E) Immunofluorescent staining for insulin (under 20 mM glucose condition) and Pdx-1 (bar 50 μm). (F) Western blot and quantitative analysis of PDX-1 protein level after 3 days of culture (*P<0.05).

Abbreviations: DAPI, 4,6-diamidino-2-phenyllindile; GSIS, glucose-stimulated insulin secretion; Glu, glucose.