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. 2015 May 18;10(5):e0127278. doi: 10.1371/journal.pone.0127278

Fig 4. Francisella priming is dependent on K+ efflux.

Fig 4

Human monocytes were pretreated with K+ (100mM) or glybenclamide (50, 100 and 200 μM) for 30 min and then infected with F. novicida. IL-18 release was measured either 16 h post-infection (A, B) or 30 min followed by 30 min of ATP stimulation (C,D). Cell culture media, extract and pellet were immunoblotted for IL-18, caspase-1 and ASC (E). Data represent mean ± SEM, n = 3 independent experiments. * p<0.05. White bars—overnight model, black bars—rapid priming model.