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. 2015 May 18;10(5):e0126558. doi: 10.1371/journal.pone.0126558

Fig 2. S5a and S5b bHLH genes in Gossypium.

Fig 2

A, NJ tree of S5a and S5b bHLH proteins from A. thaliana and Gossypium. The scale bar indicates the estimated number of amino acid replacements per site. The tree was constructed and tested using the alignment shown in S3 Fig. Percentage of supported bootstrap for every branch in a test of 1000 replicates is indicated. B, PCR confirmation of the genome-specific S5a and S5b bHLH genes in tetraploid cottons G. barbadense (Gb) and G. hirsutum (Gh), and their diploid progenitors G. arboreum (Ga) and G. raimondii (Gr). M, DNA marker with two bands of 250 bp (upper) and 100 bp (lower) indicated. C, Gene structures of cotton S5a and S5b bHLH genes. The sequences corresponding to the ORFs are depicted proportionally. Exons and introns are represented by blue bars and black lines, respectively. Red triangle indicates the long terminal repeat (LTR) retrotransposon inserted in GhbHLH062A (Details are shown in S4 Fig). For GobHLH130s, the simple sequence repeats (GAA)n of various lengths in the 6th exon are presented in green and a black triangle directs the site of a 15-bp deletion in GhbHLH130D (see details in S5 Fig).