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. 2015 May 18;10(5):e0126019. doi: 10.1371/journal.pone.0126019

Fig 2. Impact of breastfeeding duration on T-lymphocyte subsets at 6 months of age.

Fig 2

A, Gating strategy for T-cell subset delineation. CD4+ and CD8+ T-cell subsets were defined within total CD3+ T cells, and subsequently dissected using CD45RO and CCR7 into naive, central memory, CD45RO+ effector memory (TemRO) and CD45RO- effector memory (TemRA) T cells. The reference depict density plots of total lymphocytes and were used to set the gates accordingly. B, Backtransformed CD4 and CD8 cell counts (cells/μL) at 6 months of age according to different breastfeeding duration categories (reference category is never). Categories of breastfed children contain both partial and exclusively breasted children. C, The estimated backtransformed regression line reflects CD4 and CD8 cell counts (cells/μL) at 6 months of age, per month increase in breastfeeding duration. *, P<0.05.