Table 1. List of the different classes of transcripts coding for antimicrobial proteins retrieved from S. exigua transcriptome [21] and expressed in larval midgut.
| Type of transcript | n of unigenes in S. exigua transcriptome (with complete ORF) | ORF size (bp) | aa mature protein/pro-protein | Molecular Mass (kDa) | Best Blastp hit a | E-value |
|---|---|---|---|---|---|---|
| Glycine-rich AMPs | ||||||
| SeGloverin | 10 (3) | 528 | 157 | 17.4 | S. exigua ADL27731 | 3e-109 |
| SeAttacin1 | 23 (3) | 765 | 237 | 25.9 | Trichoplusia ni P50725 | 1e-105 |
| SeAttacin2 | 4 (4) | 708 | 219 | 24.2 | Heliothis virescens ACR78454 | 5e-97 |
| SeAttacin3 | 6 (2) | 708 | 219 | 24.2 | H. virescens ACR78454 | 3e-82 |
| Cysteine-rich AMPs | ||||||
| SeDefensin (SeSpodoptericin) | 8 (1) | 309 | 78 | 8.5 | S. exigua AEW24427 | 5e-50 |
| SeGallerimycin | 5 (2) | 228 | 55 | 6.2 | S. exigua ADJ95798 | 1e-31 |
| SeX-tox | 6 (0) b | 1470 b | 472 | 52.7 | S. frugiperda AFC87713 | 0.00 |
| SeCobatoxin A | 12 (4) | 261 | 69 | 7.7 | S. frugiperda AAQ18900 | 3e-46 |
| SeCobatoxin B | 4 (3) | 375 | 108 | 11.6 | T. ni ABV68874 | 2e-14 |
| SeDiapausin A1 | 4 (4) | 192 | 40 | 4.5 | Spodoptera litura ABU96713 | 3e-21 |
| SeDiapausin A2 | 2 (2) | 192 | 40 | 4.5 | S. litura ABU96713 | 6e-22 |
| SeDiapausin A3 | 2 (2) | 192 | 40 | 4.5 | S. litura ABU96713 | 6e-22 |
| SeDiapausin A4 | 2 (2) | 192 | 40 | 4.5 | S. litura ABU96713 | 6e-22 |
| SeDiapausin A5 | 2 (1) | 192 | 40 | 4.5 | S. litura ABU96713 | 6e-22 |
| SeDiapausin A6 | 2 (2) | 192 | 40 | 4.5 | S. litura ABU96713 | 6e-22 |
| SeDiapausin A7 | 2 (1) | 192 | 40 | 4.5 | S. litura ABU96713 | 6e-22 |
| SeDiapausin B1 | 2 (1) | 192 | 40 | 4.5 | S. litura ABU96713 | 1e-17 |
| Proline-rich AMPs | ||||||
| SeLebocin1 | 4 (1) | 474 | 136 | 15.6 | Galleria mellonella ACQ99193 | 5e-46 |
| SeLebocin2 | 2 (1) | 477 | 137 | 15.4 | G. mellonella ACQ99193 | 6e-36 |
| Amphipathic peptides | ||||||
| SeCecropin A1 | 10 (4) | 192 | 41 | 4.5 | S. litura ABQ51092 | 1e-19 |
| SeCecropin A2 | 4 (2) | 192 | 41 | 4.5 | S. litura ABQ51092 | 1e-19 |
| SeCecropin B1 | 3 (3) | 189 | 40 | 4.3 | S. litura Q9XZG9 | 8e-19 |
| SeCecropin C | 8 (5) | 192 | 42 | 4.5 | Helicoverpa armigera AAX51193 | 2e-13 |
| SeCecropin D | 3 (2) | 192 | 41 | 4.5 | S. litura Q9XZH0 | 8e-20 |
| SeCecropin E | 1 (1) | 192 | 41 | 4.3 | B. mori NP_001037392 | 3e-13 |
| SeCecropin F1 | 4 (2) | 189 | 40 | 4.3 | S. litura Q9XZH0 | 1e-17 |
| SeCecropin F2 | 5 (2) | 189 | 40 | 4.3 | S. litura Q9XZH0 | 7e-18 |
| SeMoricin | 8 (3) | 204 | 45 | 4.8 | S. exigua AAT38873 | 3e-22 |
| Lysozymes | ||||||
| SeC-LYZ1A | 11 (3) | 426 | 121 | 14.0 | S. exigua AAP03061 | 2e-85 |
| SeC-LYZ1B | 2 (1) | 426 | 121 | 14.0 | S. exigua AAP03061 | 7e-85 |
| SeC-LYZ1C | 2 (1) | 426 | 121 | 14.0 | S. exigua AAP03061 | 2e-85 |
| SeC-LYZ2 | 3 (2) | 426 | 121 | 13.9 | Pseudoplusia includens AAS48094 | 3e-59 |
| SeC-LYZ3 | 1(0) c | 420 c | 120 | 13.6 | Ostrinia furnacalis AGV28584 | 4e-49 |
| SeLLP1 | 9 (2) | 552 | 157 | 18.4 | Antheraea. mylitta ABP52098 | 2e-87 |
| SeLLP2 | 4(1) | 579 | 171 | 20.0 | Danaus plexippus EHJ75441 | 2e-50 |
aBlastp search performed on August 2014.
bNo unigenes with homology to pre-pro-protein x-tox including a complete ORF were found. Complete ORF was retrieved by assembling the six unigenes with Seqman software (Lasergene, DNASTAR Inc.).
cC-LYZ3 cluster includes only one unigene that does not contain a complete ORF. Searches with Blastn tools retrieved a complementary sequence (GenBank acc. nr. GAFU01015304) that was further assembled with the c-LYZ3 sequence of the present work to obtain a consensus sequence containing a full-length ORF. The existence of the full length transcript was confirmed by PCR and sequencing.