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. 2015 May 18;10(5):e0127414. doi: 10.1371/journal.pone.0127414

Fig 4. WNT/Frizzled signaling was impaired in DKC1 mutant iPS cells.

Fig 4

A: Validation of the results from microarray. LGR5, DKK1, WLS and FRZB mRNA expression were measured by real-time RT/PCR. 3 independent experiments were performed and the error bars show standard deviation. TERT CP: iPS cells with TERT R537H/2173-2187del15insACAG compound homozygotes mutation * p<0.01 between WT iPS cells and different mutant iPS cells, respectively. B: Western blot result of dyskerin protein in HEK293T cells showing doxycycline inducible DKC1 shRNA can reduce the dyskerin level to about 10–20%. shGFP was used as a control. C: Telomerase activity of HEK293T cells after knocking down of dyskerin protein from B was measured by TRAP assay. The quantitive data, derived by densitometry, are shown. D; Knock down of dyskerin protein can directly decrease the expression of LGR5, WLS and FRZB genes. Two different DKC1 shRNA lines were used in this experiment. The combined results of 3 independent experiments are shown, the error bars show standard deviation. * p<0.01.