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Effect of TGF-β blockade on renal Th17/Treg responses modulated by CCN2(IV). The main markers for Th17 or Treg were evaluated in renal total protein extracts. (a) Renal levels of IL-17A were evaluated by ELISA. (b) Foxp3 renal levels were analysed by western blot. Data is shown as ratio of renal Foxp3/GAPDH protein. Mean ± SEM of 10 mice per group and representative western blot experiment. ∗ P < 0.05 versus control.
