Table 3.

The effect of propidium iodide (PI) concentration on the 2C-value estimates of four Primulina species.

PI Conc. (μg/ml)	P. linearifolia					P. huaijiensis
	Mean PI fluorescence		Mean 2C-value (pg) ± SD	Mean RSE (%) sample	Mean RSE (%) standard	Mean PI fluorescence		Mean 2C-value (pg) ± SD	Mean RSE (%) sample	Mean RSE (%) standard
	Sample	Standard				Sample	Standard
10	48.50	81.73	1.104 ± 0.048a	0.245	0.153	49.98	89.57	1.106 ± 0.030a	0.169	0.123
25	51.05	91.74	1.140 ± 0.018ac	0.172	0.110	54.11	96.90	1.106 ± 0.034a	0.156	0.112
50	57.85	97.86	1.158 ± 0.020bc	0.178	0.118	58.62	104.36	1.112 ± 0.030a	0.152	0.117
100	62.89	105.65	1.166 ± 0.002bc	0.150	0.106	63.04	111.38	1.120 ± 0.02a	0.147	0.117
150	65.60	109.99	1.170 ± 0.002bc	0.152	0.102	65.74	115.70	1.126 ± 0.028a	0.147	0.117
200	68.76	114.34	1.178 ± 0.002bc	0.152	0.096	67.37	119.09	1.120 ± 0.020a	0.140	0.116
Mean	59.11	100.22	1.154 ± 0.014			59.81	106.17	1.114 ± 0.028
PI CONC. (μg/ml)	P. heterotricha					P. liguliformis
	Mean PI fluorescence		Mean 2C-value (pg) ± SD	Mean RSE (%) sample	Mean RSE (%) standard	Mean PI fluorescence		Mean 2C-value (pg) ± SD	Mean RSE (%) sample	Mean RSE (%) standard
	Sample	Standard				Sample	Standard
10	54.68	37.16	1.336 ± 0.048a	0.198	0.234	64.90	40.13	1.460 ± 0.056a	0.124	0.183
25	72.83	49.43	1.330 ± 0.034a	0.189	0.185	68.86	41.72	1.488 ± 0.012a	0.118	0.155
50	64.14	42.65	1.356 ± 0.044a	0.171	0.171	75.46	44.85	1.518 ± 0.044a	0.114	0.154
100	70.21	47.33	1.338 ± 0.032a	0.164	0.163	81.23	47.88	1.532 ± 0.034a	0.105	0.157
150	59.29	38.65	1.384 ± 0.054a	0.171	0.154	90.03	52.50	1.546 ± 0.080a	0.113	0.148
200	75.39	51.35	1.324 ± 0.028a	0.165	0.144	85.18	50.92	1.510 ± 0.028a	0.108	0.133
Mean	66.14	44.43	1.344 ± 0.040			77.61	46.33	1.508 ± 0.036

Letters shared (within each species) indicated no significant differences in mean 2C-value (Tukey's HSD test P = 0.05). SD, Standard deviation. Each value is the mean of four replicates.